Recent advances in two-photon microscopy for studying cardiovascular diseases
Two-photon excitation (TPE) microscopy has become an important branch in fluorescent microscopy to study high-speed biological events in vivo with high penetration depth, i.e., 600 – 800 microns; although this is still considered “shallow” in many people’s view for studying cardiovascular diseases (CVDs), it can be readily applied to investigate CVDs on various animal models with unique capabilities, e.g., real-time 3D imaging with subcellular resolution and molecular specificity etc., which cannot be found in conventional medical imaging systems, e.g., magnetic resonance imaging. In this seminar, I will first review the basic principle of TPE microscopy and its recent applications in studying CVDs; next, I will present our recent work on TPE microscopy, including the development of the first digital-micromirror device (DMD)-TPE microscope system based on binary holography which enables multi-focus random-access scanning at 22.7 kHz as well as the implementation of the sensorless adaptive optics algorithm to our DMD system for realizing deep brain imaging. Lastly, I will present the development of a two-photon tomography system, consisting of a TPE microscope and a custom-designed high-precision vibrating microtome, which can generate volumetric image of an entire organ, such as a mouse brain, heart or liver, with subcellular resolution.
Dr. Shih-Chi Chen is a Professor in the Department of Mechanical and Automation Engineering at the Chinese University of Hong Kong. He received his B.S. degree in Mechanical Engineering from the National Tsing Hua University, Taiwan, in 1999; and his S.M. and Ph.D. degrees in Mechanical Engineering from the Massachusetts Institute of Technology, Cambridge, in 2003 and 2007, respectively. Following his graduate work, he entered a post-doctoral fellowship in the Wellman Center for Photomedicine, Harvard Medical School, where his research focused on biomedical optics and endomicroscopy. From 2009 to 2011, he was a Senior Scientist at Nano Terra, Inc., a start-up company founded by Prof. George Whitesides at Harvard University, to develop precision instruments for novel nanofabrication processes. His current research interests include ultrafast laser applications, biomedical optics, precision engineering, and nanomanufacturing. Dr. Chen is a member of the American Society for Precision Engineering (ASPE), American Society of Mechanical Engineers (ASME), SPIE, and the Optical Society (OSA); and currently serves as the Associate Editor of ASME Journal of Micro- and Nano-Manufacturing, IEEE Transactions on Nanotechnology, and HKIE Transactions. In 2003 and 2018, he received the prestigious R&D 100 Awards for developing a six-axis nanopositioner and an ultrafast nanoscale 3-D printer respectively.